Comparative efficiency of five potash and phosphate solubilizing bacteria and their key enzymes useful for enhancing and improvement of soil fertility sajidmohammad a saiyad, dr. Symbionk is a biofertilizer based on a selective strain of potash solubilizing, beneficial bacteria frateuria aurantia. Differences in distribution of potassiumsolubilizing. Results showed that rock p and k materials either applied singly or in combination did not significantly enhance soil availability of p and k. A total of fifty two bacterial strains were isolated, characterized and classified to twenty two nfb, eighteen psb and twelve ksb. Phosphate solubilizing bacteria and fungi play an important role in p bioavailability and can be used to increase pue bolan et al. Potassium is one of the essential nutrients for plant to growth and required in large amount to achieve optimum growth. Quality control appearance cream to yellow homogeneous free flowing powder gelling firm, comparable with 2.
The bacteria and fungi convert nonlabile p through either inorganic psolubilizing or organic p mineralization processes. Thus, application of k solubilizing bacteria as biofertilizer for agriculture improvement can reduce the use of. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Download pdf potassium nutrition ebook free online. Potassiumsolubilizing bacteria and their application in.
They convert insoluble potassium in the soil into a form that plants can access. The morphological and the biochemical characteristics of the 5 good potassium solubilizers are presented in tables 2. Few studies, however, have focused on communities of bacteria able to solubilize inorganic phosphate ipsb. Results showed that rock p and k applied either singly or in combination did not signi. Potassium solubilizing bacteria will grow on this medium and form a clear zone around the colony, formed due to potassium solubilization in the vicinity of the colony. Dec 12, 2018 liquid biofertiliser useful in all crops including legumes. Experiments were conducted to evaluate the potential of phosphate solubilizing bacteria psb bacillus megaterium and potassium solubilizing bacteria ksb bacillus mucilaginosus inoculated in nutrient limited soil planted with eggplant. Pdf potassium solubilizing bacteria ksb researchgate. Potassium solubilizing microorganisms for sustainable agriculture. Genome announcement potassium k is an important macronutrient for plant growth and development, and k is a limited element due to its lesser availability in soluble form in. Apr 27, 2017 these data provide the basis to investigate the relative impact of bacteria in feldspar solubilizing and the molecular mechanism of the potassium feldspars dissolution. The utilization of microbes including phosphate solubilizing bacteria psb and potassium solubilizing bacteria ksb is deemed as an alternative to address. Jun 22, 2016 pgp bacteria isolation and determinations of n 2fixing, p and ksolubilizing ability. Potassium solubilizing microorganisms for sustainable.
This is a promising strategy for the improvement of plant absorption of potassium and so reducing the use of chemical fertilizer. Number of microbial species plays key role in p solubilization these includes bacteria, fungi, actinomycetes and even algae. These microbial isolates showed potassium solubilization index from 1. Isolation of potassium solubilizing bacteria a total of 37 potassium solubilizing bacteria isolates were obtained from limestone quarry area soil samples of pt indocement tbk, palimanan cirebon using aleksandrov medium. Biofertilizer is a good platform to deliver this primary macronutrient by assistance of potassium solubilizing bacteria ksb.
Occurrence of phosphate solubilizing bacteria high proportion of psm is concentrated in the rhizosphere, and they are metabolically more active than from other sources vazquez et al. Isolation, screening and characterization of phosphate. Phosphate solubilizing bacteria psb english youtube. Psb possess phosphate solubilizing ability and they can. Usually, one gram of fertile soil contains 10 1 to 10 10 bacteria, and their live weight may exceed 2,000 kg ha1. Diameter of halo zone was measured after 42 hours, up to 15 days. Phosphorous and phosphate solubilising bacteria and their. The utilization of potassium solubilizing bacteria ksb to increase the soluble k content in soil has been regarded as a desirable pathway to increase plant yields. To acquire the optimum outcome by applying this bacterium into the biofertilizer. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. Isolation and identification of potassiumsolubilizing. Some types of bacteria and fungi that have this ability include pseudomonas, mycobacterium, micrococcus, flavobacterium, penicillium, sclerotium, bacillus and thiobacillus bacteria and nitrosomonas 6.
To obtain the optimum ph on the potassium solubilizing bacteria growth within stated range. Download potassium nutrition pdf epub, mobi ebooks without registration on our website. Potassium k is the third major essential macronutrient for plant growth. Differences in distribution of potassiumsolubilizing bacteria. Selection and identification of phosphatepotassium solubilizing. The biochemical characters are studied for the five isolates of potassium solubilizing bacteria presented in. Phosphatesolubilizing bacteria nullify the antagonistic. Materials and methods preparation of bacteria and medium the phosphates solubilizing bacteria were isolated from oxisols in central java, indonesia phh 2 0 5. Atmospheric nitrogen fixation, free living nitrogen fixing bacteria, phosphate solubilizing bacteria, phosphate. Effect of coinoculation with phosphate and potassium solubilizing. Responses to soil ph gradients of inorganic phosphate. Zinc solubilizing agar m2023 intended use recommended for growth and maintenance of zinc solubilizing soil microorganisms. Effect of coinoculation with phosphate and potassium.
Molecular characterization of potassium solubilizing bacteria. Owing to naturally available source of potassium in soil and high price of synthetic potassium fertilizers, the importance of ksb is increasing day by day. Beneficial bacteria activate nutrients and promote wheat. Two phosphate and potassiumsolubilizing strains knp4 and. Both isolates effectively dissolved mineral phosphate and potassium, while strain knp414 showed higher dissolution capacity even than bacillus mucilaginosus as1. Instant access to millions of titles from our library and its free to try. Potassium is a vital component of plant nutrition package limiting crop yield and. Pgp bacteria isolation and determinations of n 2fixing, p and ksolubilizing ability. Jan 10, 2019 soil ph is commonly considered a dominant factor affecting the function of microbiota. Potassium solubilizing bacterial strains resulted in release of 35. To obtain the optimum temperature on the potassium solubilizing bacteria growth within stated range. Potassium solubilisation and identification of best solubilizer the all five isolates were grown on k enriched 100 ml broth containing 1% glucose, 0. Ksolubilization is carried out by a large number of saprophytic bacteria bacillus mucilaginosus, b.
Plantgrowthpromoting rhizobacteria are freeliving soil microorganisms that inhabit. A complex inoculant of n2fixing, p and ksolubilizing. Composition ingredients gms litre dextrose glucose 10. Potassium k has been recognized as an essential element in intensive agricultural production systems, and deficiency of k usually results in a decrease in crop yields. Isolation and identification of n fixing, phosphate and 2. Secretion of organic acids by phosphate solubilizing bacteria. Symbionk, biofertilizer, potash solubilizing, bacteria. Potassium solubilization in phonolite rock by diazotrophic. Badr41 studied potassium and phosphorus solubilization capacity of silicate solubilizing bacteria and it ranged from 490 mg to 758 mg l1 at ph 6. Gas chromatographic analyses of cell free spent culture medium from the various bacteria demonstrated the presence of 11 identified, and several unidentified, volatile and nonvolatile organic acids. The concentrations of soluble potassium in the soil are usually very low and more than 90% of potassium in the soil exists in the form of insoluble rocks and silicate minerals. Qualitative estimation of all the suspected phosphate solubilizing bacteria were screened by inoculating and growing on pvk medium and nbrip medium with bromophenol blue.
Phosphatesolubilizing bacteria and fungi play an important role in p bioavailability and can be used to increase pue bolan et al. Phosphate solubilizing bacteria are able to solubilize phosphate and made it available for plants by solubilizing the organic acids. Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays. Potassium solubilizing bacteria biofertilizer mineral bearing potassium. This procedure was repeated five times and the obtained suspensions from each plot were mixed and then diluted with. Fifteen isolates had efficient potassium solubilizing ability, of which k. Request pdf mineralization of phosphorous by phosphate solubilizing microbes affects soil fertility organic farming in the coming decades is attracting the consideration of researchers and. Phosphate and potassium solubilizing bacteria effect on. All books are in clear copy here, and all files are secure so dont worry about it.
Comparative efficiency of five potash and phosphate. Phosphate solubilizing bacteria an overview sciencedirect. Following the inoculation of ksb in the soil, potassium can. The potassium solubilizing microorganisms ksms are a rhizospheric.
Potassium solubilizing bacteria ksb could serve as inoculants. Pdf perspectives of potassium solubilizing microbes in. Isolation and characterization of phosphatesolubilizing. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants through release from inorganic and organic soil p pools by solubilization and mineralization. The potassium solubilizing bacteria isolates were characterized by a clear zone that formed around the colony figure 1. Pdf potassium is a major essential plant nutrient that plays a. Occurrence, mechanisms and their role in crop production ahmad ali khan 1, ghulam jilani 1, mohammad saleem akhtar 1, syed muhammad saqlan naqvi 2, mohammad rasheed 3 abstract plants acquire phosphorus from soil solution as phosphate anion. The potassium solubilizing microorganisms ksms are a rhizospheric microorganism which solubilizes the insoluble potassium k to soluble forms of k for plant growth and yield. Potassium solubilizing bacteria ksb mechanisms, promotion. Evidence for naturally occurring phosphate solubilizing microorganism dates back to 1903 khan et al. Isolation of phosphate solubilizing bacteria and their ability to solubilize calcium phosphate from the rhizosphere soils tomato, jasmine, onion, and maize, the total of 12 bacterial species of 32 samples were found enriched on pikovskayas agar plates. Phosphate solubilizing microorganisms naturally occurring rhizospheric phosphorus solubilizing microorganism psm dates back to 1903 khan et al. Jun 28, 2016 these potassium solubilizing bacteria ksb can be used as a promising approach to increase k availability in soils, thus playing an important role for crop establishment under klimited soils. Nov 23, 2017 phosphate solubilizing bacteria psb reduce the negative effects of soil calcification on soil phosphorus p nutrition.
Two phosphate and potassiumsolubilizing bacteria isolated. Organic acids produced by potassium solubilizing microbes. May 14, 2006 two phosphate and potassium solubilizing strains knp4 and knp414 were isolated from the soil of tianmu mountain, zhejiang province china and they were phenotypically and phylogenetically characterized. Culturable total and beneficial microbial occurrences in long.